Studies of the biosynthesis of the chondroitin sulfate-protein complex by Alvin Telser

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LC ClassificationsMicrofilm 20489
The Physical Object
FormatMicroform
Paginationx, 157 l.
Number of Pages157
ID Numbers
Open LibraryOL1368696M
LC Control Number92895996

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Perlman rl, telser a, dorfman a. the biosynthesis of chondroitin sulfate by a cell-free preparation. j biol chem. nov; – silbert je. incorporation of 14c and 3h from labeled nucleotide sugars into a polysaccharide in the presence of a cell-free preparation from cartilage. Studies on the biosynthesis of the chondroitin sulfate-protein linkage region.

Journals & Books; Register Sign in. Sign in Register. Vol Issue 5, 10 JunePages Studies on the biosynthesis of the chondroitin sulfate-protein linkage region. Author links open overlay panel Torsten Helting a b c Lennart Rodén a b c Cited by: Robinson HC, Telser A, Dorfman A.

Studies on biosynthesis of the linkage region of chondroitin sulfate-protein complex. Proc Natl Acad Sci U S A. Dec; 56 (6)– [PMC free article] Helting T, Rodén L. Studies on the biosynthesis of the chondroitin sulfate-protein linkage region. Biochem Biophys Res by: Discussion The findings described above together with earlier studies (Robinson et al., ), provide evidence for the biosynthesis of the two galactose residues (Reactions II and III) in the protein-carbohydrate linkage region of chondroitin by:   The biosynthesis of chondroitin-sulfate protein complex A Telser, H C Robinson, A Dorfman Proceedings of the National Academy of Sciences Sep54 (3) ; DOI: /pnasCited by: In the native state, chondroitinsulfate is covalently bound to protein.

Since the original studies of 1\4uir,l which implicated serine in this linkage, Roden et al. in this laboratory have demonstrated that the linkage region between protein and polysaccharide in heparin,2 chondroitinsulfate,3' 4 and dermatan sulfate5 has the.

The biosynthesis of chondroitin-sulfate protein complex Article (PDF Available) in Proceedings of the National Academy of Sciences 54(3) October with 22 Reads How we measure 'reads'.

35S04= into chondroitin sulfate, a number of investigators have demonstrated the incorporation of 35S04= by way of 3’-phos- phoadenosine 5’-phosphosulfate into various sulfated mucopoly- saccharides (5, 6).

In none of these studies has evidence of synthesis of. Telser A, Robinson HC, Dorfman A. The biosynthesis of chondroitin-sulfate protein complex. Proc Natl Acad Sci U S A. Sep; 54 (3)– [PMC free article] O'Brien PJ, Canady MR, Hall CW, Neufeld EF.

Transfer of N-acetylneuraminic acid to incomplete glycoproteins associated with microsomes. Biochim Biophys Acta. Apr 25; (2) Cited by: Previous studies have shown that D-xylose partially overcomes the puromycin inhibition of chondroitin sulfate synthesis in cultured chick embryo chondrocytes.

Likewise, D-xylose stimulates chondroitin sulfate synthesis by limb bud mesenchyme cells previously treated with BrdU or limb bud cartilage cells treated with by: Biosynthesis of chondroitin sulfate side chains and hyaluronate time-course studies with cartilage slices Studies of the biosynthesis of the chondroitin sulfate-protein complex book calf ribs under different conditions.

Biochimica et Biophysica Acta (BBA) - General Subjects(2), DOI: /(76) Norman Kharasch, Ajit S. Arora. Studies on the metabolism of the chondroitin 4-sulfate-protein complex of porcine articular cartilage Author links open overlay panel N.

Katsura a E.A. Davidson b Show moreCited by: 3. by Smith degradation of bovine chondroitin sulfate-protein complex essentially as described by Baker et al.

(14). De- sulfated Ch-S, a gift from Dr. Cifonelli (University of Chicago), was prepared from 5 g of chondroitin sulfate by the method of Kantor and Schubert (16).

ChS hexa and ChSCited by: Studies on biosynthesis of the linkage region of chondroitin sulfate-protein complex Article (PDF Available) in Proceedings of the National Academy of Sciences 56(6) January with.

The biosynthesis of chondroitin-sulfate protein complex. [Studies on the biosynthesis of chondroitin sulfate proteins. Characterization of the protein polysaccharides from calf costal cartilage and their labelling in vitro with divalent 35S-sulfate] Kleine TO, Hilz H.

Introduction. Chondroitin sulfate (CS) is a representative sulfated glycosaminoglycan (GAG) that is widespread on cell surfaces and within extra/pericellular matrices in the form of proteoglycans (CSPGs), where at least one CS side chain is covalently attached to a panel of core by: Biosynthesis of the chondroitin sulfate proteoglycan.

Purification and properties of xylosyltransferase Article (PDF Available) in Journal of Biological Chemistry (11) July with. Studies on the metabolism of the chondroitin 4-sulfate-protein complex of porcine articular cartilage.

Katsura N, Davidson EA. Biochim Biophys Acta, (3), 01 Sep Cited by: 2 articles | PMID: Cited by: 1. Telser A, Robinson HC, Dorfman A. The biosynthesis of chondroitin-sulfate protein complex. Proc Natl Acad Sci U S A.

Sep; 54 (3)– [Europe PMC free article] [Google Scholar] O'Brien PJ, Canady MR, Hall CW, Neufeld EF. Transfer of N-acetylneuraminic acid to incomplete glycoproteins associated with microsomes. Biochim Biophys Acta. The biosynthesis of chondroitin sulphates influence of nucleotides and hexosamines on sulphate incorporation.

Biochimica et Biophysica Acta51 (3), DOI: /(61) Konoshin ONODERA, Tohru by: Bowness (9) has pre- sented evidence that the fractionation of cartilage by a mild treatment, such as that used in this study, yields chondroitin sulfate in an apparent homogeneous complex with protein. The chondroitin sulfate fraction of chick embryo cartilage was presumed to be present in the form of a protein complex, and 90 HALVERSON, HILLS, AND WHITEHEAD TABLE II EXTENT OF THE Cited by: 5.

Biosynthesis of Chondroitin Sulfate Proteoglycan Article (PDF Available) in Journal of Biological Chemistry (12) June with 16 Reads How we measure 'reads'. studies on biosynthesis of the linkage region of chondroitin sulfate-protein complex* By H.

Robinson, Alvin Telser and Albert Dorfman Topics: Biological Sciences: Biochemistry. Robinson HC, Telser A, Dorfman A. Studies on biosynthesis of the linkage region of chondroitin sulfate-protein complex. Proc Natl Acad Sci U S A. Dec; 56 (6)– [PMC free article] Articles from Biochemical Journal are provided here courtesy of The Biochemical by: Robinson HC, Telser A, Dorfman A.

Studies on biosynthesis of the linkage region of chondroitin sulfate-protein complex. Proc Natl Acad Sci U S A. Dec; 56 (6)– [PMC free article] Telser A, Robinson HC, Dorfman A.

The biosynthesis of chondroitin sulfate. Arch Biochem Biophys. Sep 26; (1)– the biosynthesis of chondroitin sulfate-protein complex carried out with a preparation from la-day-old embryonic chick epi- physeal cartilage () clarified the mechanism of alternation of monosaccharides in chondroitin sulfate chains.

These studies indicated that the mechanism of chondroitin. Biosynthesis of Chondroitin Sulfate INDEPENDENT ADDITION OF GLUCURONIC ACID AND N-ACETYLGALACTOSAMINE TO OLIGOSACCHARIDES* (Received for publication, Novem ) JEREMIAH E. SILBERT$ AND ALBERT C. REPPUCCI, JR.$ From the Connective Tissue Research Laboratory, Boston Veterans Administration Hospital, and the.

Purchase Chondroitin Sulfate, Volume 53 - 1st Edition. Print Book & E-Book. ISBNBook Edition: 1. Biosynthesis of the acetylgalactosamine 4,6-disulfate unit of squid chondroitin sulfate by transsulfation from 3'phosphoadenosine 5'phosphosulfate.

January Journal of Biological Chemistry Allen C. Stoolmiller, Allen L. Horwitz, Albert DorfmanCited by: Early interest in the biosynthesis of glycosaminoglycans was generated by the discovery of lysosomal storage diseases and disorders of connective tissue metabolism such as arthritis.

Keywords Hyaluronic Acid Heparan Sulfate Chondroitin Sulfate Core Protein Sulfate ProteoglycanCited by: 6. A new study has confirmed, no glucosamine, no problem. Chondroitin Sulfate (CS) has been shown to be effective for joint pain. It’s been proven as effective as.

Chondroitin sulfate is a sulfated glycosaminoglycan (GAG) composed of a chain of alternating sugars (N-acetylgalactosamine and glucuronic acid).It is usually found attached to proteins as part of a proteoglycan.A chondroitin chain can have over individual sugars, each of which can be sulfated in variable positions and quantities.

With the possible exception of hyaluronic acid, the connective tissue polysaccharides are all synthesized by their parent cells as components of proteoglycans. In these substances, a number of Cited by: This chapter reviews recent progress concerning the biosynthesis of glycosaminoglycans in nervous tissue.

E.,Studies on the biosynthesis of chondroitin sulfate, Doctoral dissertation, The University of Michigan, Ann Arbor. E., Distler, J., and Jourdian, G. W.,Biosynthesis of the chondroitin sulfate- protein linkage Cited by: 7. Abstract. Seventy-five years after its discovery (1,2), heparin remains an important tool in medicine.

Among its established uses are the prevention of postoperative thrombosis, the treatment of acute venous thrombosis, and the prevention of clot formation in the heart-lung machine (3).Cited by: 7 GLYCOPROTEIN BIOSYNTHESIS - I Studies with intact cells BIOSYNTHESIS Biosynthesis of chondroitin sulfate 2 83 Xylosyl transfer As an introduction to the course, I strongly recom­ COMPLEX CARBOHYDRATES.

COMPLEX CARBOHYDRATES. COMPLEX Size: 2MB. Morphogenetic Role of Glycosaminoglycans (Acid Mucopolysaccharides) in Brain and Other Tissues Telser, A., and Dorfman, A.,Studies on biosynthesis of the linkage region of chondroitin sulfate-protein complex, Proc.

Natl. Acad Toole B.P. () Morphogenetic Role of Glycosaminoglycans (Acid Mucopolysaccharides) in Brain and Other Cited by: These studies led to the development of quantitative methods for assays of hyaluronidase (), discovery that chondroitin sulfate was a substrate for testicular hyaluronidase (), and recognition that hyaluronidase was unusually stable to heat and acid pH (), special properties that were later recognized as those of lysosomal enzymes.

There is a history of clinical research done on glycosaminoglycans, especially glucosamine and chondroitin, for the treatment of glucosamine is a precursor for glycosaminoglycans, and glycosaminoglycans are major components of cartilage, ingesting glucosamine might nourish joints, and thereby alleviate arthritis symptoms.

Some of the evidence for the effectiveness of. completed polysaccharide. Recent studies on formation of glycoproteins in rat liver"l indicate that these compounds are synthesized by the first of the three mechanisms.

The experiments reported here were designed to test which of these mechanisms obtains in the biosynthesis of chondroitin sulfate-protein complex. Abstract. Chondroitin sulfate (CS) is a polysaccharide consisting of repeating disaccharide units of N-acetyl-d-galactosamine and d-glucuronic acid residues, modified with sulfated residues at various date six glycosyltransferases for chondroitin synthesis have been identified, and the complex of chondroitin sulfate synthase-1 (CSS1)/chondroitin synthase-1 (ChSy-1) and chondroitin.

This book provides a comprehensive, up-to-date review of the structure, biology, and molecular interactions of proteoglycans, consolidating into a single source research using molecular, cellular, and animal systems. Of particular note is an in-depth look at the role of heparan sulfate in modulating growth factors and morphogens.

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